What is the catalog number for PLK1 Antibody?

The catalog number for PLK1 Antibody is RQ5512.

What sizes are available for PLK1 Antibody?

PLK1 Antibody is available in the following size(s): 100 µg.

Is PLK1 Antibody in stock?

PLK1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

PLK1 Antibody

Serine/threonine-protein kinase PLK1, also known as polo-like kinase 1 (PLK-1) or serine/threonine-protein kinase 13 (STPK13), is an enzyme that in humans is encoded by the PLK1 (polo-like kinase1) gene. Plk1 is an early trigger for G2/M transition. It supports the functional maturation of the centrosome in late G2/early prophase and establishment of the bipolar spindle. Also, Plk1 phosphorylates and activates cdc25C, a phosphatase that dephosphorylates and activates the cyclinB/cdc2 complex. Studies have shown that the loss of PLK1 expression can induce pro-apoptotic pathways and inhibit growth. Based on yeast and murine studies of meiosis, human PLK1 may also have a regulatory function in meiosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P53350

Host

Mouse

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

A human recombinant protein (amino acids K86-N430) was used as the immunogen for the PLK1 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

6D11

Applications

WB, IHC-P

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the PLK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PLK1 antibody is available for research use only.

Storage Conditions

After reconstitution, the PLK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PLK1 antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE mouse intestine with PLK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.