IRS1 Antibody / Insulin receptor substrate 1

Insulin receptor substrate 1 (IRS-1) is a signalling adapter protein that in humans is encoded by the IRS1 gene. It is mapped to 2q36.3. This gene exhibited no intrinsic enzyme activity, and it can serve as a docking protein involved in binding and activating other signal transduction molecules after being phosphorylated on tyrosine by insulin receptor kinase. IRS1 plays a key role in transmitting signals from the insulin and insulin-like growth factor-1 (IGF-1) receptors to intracellular pathways PI3K/Akt and Erk MAP kinase pathways. IRS1 also has important biological function for both metabolic and mitogenic (growth promoting) pathways. In addition to those, IRS1 is a key regulator of PI3K within malignant cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence/Immunocytochemistry: 2-4 µg/mL

UniProt

P35568

Host

Mouse

Reactivity

Human

Immunogen

A human recombinant protein (amino acids S1041-Q1242) was used as the immunogen for the IRS1 antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

10I3

Applications

WB, IHC-P, FACS, IF/ICC

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the IRS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This IRS1 antibody is available for research use only.

Storage Conditions

After reconstitution, the IRS1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the IRS1 antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human placenta with IRS1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.