Hsp27 Antibody

HSPB1 (Heat shock 27kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. HSP27 gene is mapped to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN) .

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunocytochemistry/Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P04792

Host

Mouse

Reactivity

Human

Immunogen

A human recombinant protein (amino acids M1-K205) was used as the immunogen for the Hsp27 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

3H3

Applications

WB, IHC-P, ICC/IF, FACS

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Hsp27 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Hsp27 antibody is available for research use only.

Storage Conditions

After reconstitution, the Hsp27 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Hsp27 antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human U-2 OS cells with Hsp27 antibody (green) and DAPI (blue) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.