BAK1 Antibody

BAK, officially called Bcl2 antagonist killer, is a protein that in humans, encoded by the BAK gene. The BAK protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAK gene spans 7.6 kb and contains 6 exons. By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, BAK gene is localized to chromosome 6. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunocytochemistry/Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q16611

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant protein (amino acids A22-S211) was used as the immunogen for the BAK1 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

4C2

Applications

WB, IHC-P, ICC/IF, FACS

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the BAK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This BAK1 antibody is available for research use only.

Storage Conditions

After reconstitution, the BAK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the BAK1 antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human A431 cells with BAK1 antibody (green) and DAPI (blue) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.