Cofilin 2 Antibody / CFL2

Cofilin 2 (muscle), also known as CFL2, is a protein which in humans is encoded by the CFL2 gene. It is mapped to 14q12. This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. And this protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunocytochemistry/Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q9Y281

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids KDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKL were used as the immunogen for the Cofilin 2 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

8C13

Applications

WB, IHC-P, ICC/IF, FACS

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Cofilin 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Cofilin 2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Cofilin 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Cofilin 2 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of FFPE human U-2 OS cells with Cofilin 2 antibody (green) and DAPI (blue) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.