EpCAM Antibody

The EPCAM gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE) : 1:50-1:200, Immunofluorescence: 1:50

UniProt

P16422

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide specific to human EpCAM was used as the immunogen for the EpCAM antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

CIB-5

Type

Recombinant

Applications

WB, IHC-P, IF

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the EpCAM antibody at -20oC.

Limitations

This EpCAM antibody is available for research use only.

Storage Conditions

Store the EpCAM antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the EpCAM antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE mouse small intestine tissue with EpCAM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.