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Histone H1.2 Antibody / HIST1H1C

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. The HIST1H1C gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:2000

UniProt

P16403

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide specific to human Histone H1.2 / HIST1H1C was used as the immunogen for the Histone H1.2 antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

AEDF-8

Type

Recombinant

Applications

WB

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the Histone H1.2 antibody at -20oC.

Limitations

This Histone H1.2 antibody is available for research use only.

Storage Conditions

Store the Histone H1.2 antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Histone H1.2 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) human MCF7, 2) mouse NIH3T3 and 3) rat C6 cell lysate with Histone H1.2 antibody. Expected molecular weight ~21 kDa (unmodified), ~30 kDa (modified) .

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