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Phospho-CREB1 Antibody (pS133)

The CREB1 gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:200

UniProt

P16220

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide specific to human CREB (surrounding pS133) was used as the immunogen for the CREB1 antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

IG-3

Type

Recombinant

Applications

IHC-P, WB

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the phospho-CREB1 antibody (pS133) at -20oC.

Limitations

This phospho-CREB1 antibody (pS133) is available for research use only.

Storage Conditions

Store the phospho-CREB1 antibody (pS133) at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the phospho-CREB1 antibody (pS133) should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of lysate from human HeLa cells treated with Calyculin A, with phospho-CREB1 antibody (pS133) . Expected molecular weight: 37-43 kDa.

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