H2AFX Antibody / Histone H2AX
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [RefSeq]
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200
UniProt
P16104
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide specific to human Histone H2A.X / H2AFX was used as the immunogen for the H2AFX antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
BIA-8
Type
Recombinant
Applications
IHC-P, WB
Purity
Affinity purified
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the H2AFX antibody at -20oC.
Limitations
This H2AFX antibody is available for research use only.
Storage Conditions
Store the H2AFX antibody at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the H2AFX antibody should be determined by the researcher.
Location
Nuclear
Image Legend
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