H2AFX Antibody / Histone H2AX

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200

UniProt

P16104

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide specific to human Histone H2A.X / H2AFX was used as the immunogen for the H2AFX antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

BIA-8

Type

Recombinant

Applications

IHC-P, WB

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the H2AFX antibody at -20oC.

Limitations

This H2AFX antibody is available for research use only.

Storage Conditions

Store the H2AFX antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the H2AFX antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE rat lung tissue with H2AFX antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.