Semaphorin 3B Antibody / SEMA3B

Semaphorin-3B is a protein that in humans is encoded by the SEMA3B gene. It is mapped to 3p21.31. The semaphorin/collapsin family of molecules plays a critical role in the guidance of growth cones during neuronal development. The secreted protein encoded by this gene family member is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q13214

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids Y55-R624 from the human protein were used as the immunogen for the Semaphorin 3B antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

Store the Semaphorin 3B antibody at -20oC.

Limitations

This Semaphorin 3B antibody is available for research use only.

Storage Conditions

Store the Semaphorin 3B antibody at -20°C.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Semaphorin 3B antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human breast cancer with Semaphorin 3B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.