CD11b Antibody / MAC-1 / ITGAM

The ITGAM gene encodes the integrin alpha M chain, also called CD11b and MAC-1. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3') . The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:200

UniProt

P11215

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide specific to human CD11b / ITGAM was used as the immunogen for the CD11b antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

BCG-9

Type

Recombinant

Applications

IHC-P, WB

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the CD11b antibody at -20oC.

Limitations

This CD11b antibody is available for research use only.

Storage Conditions

Store the CD11b antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the CD11b antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human colon carcinoma with CD11b antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.