Osteopontin Antibody / OPN / SPP1

The protein encoded by the SPP1 gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:2000, Immunofluorescence: 1:50-1:200

UniProt

P10451

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide specific to human Osteopontin / SPP1 was used as the immunogen for the Osteopontin antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

CEO-19

Type

Recombinant

Applications

WB, IF

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the Osteopontin antibody at -20oC.

Limitations

This Osteopontin antibody is available for research use only.

Storage Conditions

Store the Osteopontin antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Osteopontin antibody should be determined by the researcher.

Image Legend

Western blot testing of human HepG2 cell lysate with Osteopontin antibody. Predicted molecular weight: 35-65 kDa depending on degree of glycosylation.