Granzyme B Antibody

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:50-1:200

UniProt

P10144

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide specific to human Granzyme B / GZMB was used as the immunogen for the Granzyme B antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

AAHC-7

Type

Recombinant

Applications

IHC-P

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the Granzyme B antibody at -20oC.

Limitations

This Granzyme B antibody is available for research use only.

Storage Conditions

Store the Granzyme B antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Granzyme B antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human T-cell lymphoma tissue with Granzyme B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.