Cleaved PARP Antibody
The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. [Wiki] Cleavage of PARP, by enzymes such as caspases or cathepsins, typically inactivates PARP. While in vitro cleavage by caspase occurs throughout the caspase family, preliminary data suggest that caspase-3 and caspase-7 are responsible for in vivo cleavage. Cleavage occurs at aspartic acid 214 and glycine 215, separating PARP into a 24kDA and 89kDA segment. The smaller moiety includes the zinc finger motif requisite in DNA binding. The 89 kDa fragment includes the auto-modification domain and catalytic domain. [Wiki]
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1:500-1:2000
UniProt
P09874
Host
Rabbit
Reactivity
Human
Immunogen
A synthetic peptide specific to human PARP (89kDa cleavage segment) was used as the immunogen for the Cleaved PARP antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
BHH-16
Type
Recombinant
Applications
WB
Purity
Affinity purified
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the Cleaved PARP antibody at -20oC.
Limitations
This Cleaved PARP antibody is available for research use only.
Storage Conditions
Store the Cleaved PARP antibody at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the Cleaved PARP antibody should be determined by the researcher.
Image Legend
Western blot testing of human Jukat cell lysate with Cleaved PARP antibody. Predicted molecular weight ~89 kDa (auto-modification/catalytic domain) .
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