HMGB1 Antibody

High mobility group protein B1 is a multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V (D) J recombination, DNA repair and genome stability. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

P09429

Host

Rabbit

Reactivity

Human, Rat

Immunogen

A synthetic peptide specific to human HMGB1 was used as the immunogen for the HMGB1 antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

AAC-8

Type

Recombinant

Applications

IHC-P, WB

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the HMGB1 antibody at -20oC.

Limitations

This HMGB1 antibody is available for research use only.

Storage Conditions

Store the HMGB1 antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the HMGB1 antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic, cell membrane, extracellular

Image Legend

IHC staining of FFPE human kidney tissue with HMGB1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.