Phospho-Vimentin Antibody (pS72)
The VIM gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. [RefSeq]
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1:500-1:2000
UniProt
P08670
Host
Rabbit
Reactivity
Human
Immunogen
A synthetic peptide specific to human Vimentin (surrounding phosphorylated serine 72) was used as the immunogen for the phospho-Vimentin antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
ADE-22
Type
Recombinant
Applications
WB
Purity
Affinity purified
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the phospho-Vimentin antibody (pS72) at -20oC.
Limitations
This phospho-Vimentin antibody (pS72) is available for research use only.
Storage Conditions
Store the phospho-Vimentin antibody (pS72) at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the phospho-Vimentin antibody (pS72) should be determined by the researcher.
Image Legend
Western blot testing of lysate from Calyculin A-treated HeLa cells with phospho-Vimentin antibody (pS72) . Expected molecular weight: 53-58 kDa.
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