Firefly Antibody

Firefly is the light-emitting enzyme responsible for the bioluminescence of fireflies and click beetles. The enzyme catalyses the oxidation of firefly luciferin, requiring oxygen and ATP. Because of the requirement of ATP, Fireflys have been used extensively in biotechnology. [Wiki]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:1000-1:5000, Immunofluorescence: 1:50-1:200

UniProt

P08659

Host

Rabbit

Reactivity

Firefly

Immunogen

A synthetic peptide specific to Firefly was used as the immunogen for the Firefly antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

DAB-6

Type

Recombinant

Applications

WB, IF

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the Firefly Luciferase antibody at -20oC.

Limitations

This Firefly antibody is available for research use only.

Storage Conditions

Store the Firefly antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Firefly antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) 293T cells transfected with Firefly, 2) human HeLa, 3) mouse NIH3T3 and 4) rat C6 lysate with Firefly antibody. Predicted molecular weight ~60 kDa.