Firefly Antibody
Firefly is the light-emitting enzyme responsible for the bioluminescence of fireflies and click beetles. The enzyme catalyses the oxidation of firefly luciferin, requiring oxygen and ATP. Because of the requirement of ATP, Fireflys have been used extensively in biotechnology. [Wiki]
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1:500-1:2000, Immunofluorescence: 1-50-1:200
UniProt
P08659, V9VFW4
Host
Rabbit
Reactivity
Firefly
Immunogen
A synthetic peptide specific to Firefly was used as the immunogen for the Firefly antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
DAA-6
Type
Recombinant
Applications
WB, IF
Purity
Affinity purified
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the Firefly Luciferase antibody at -20oC.
Limitations
This Firefly antibody is available for research use only.
Storage Conditions
Store the Firefly antibody at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the Firefly antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of human 293 cells transfected with Firefly using Firefly antibody (green) and DAPI nuclear stain (blue) .
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