ATP Citrate Lyase Antibody

ATP citrate lyase, aslo known as ACLY, is anenzymethat in animals represents an important step infatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis ofcytosolicacetyl-CoAin many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, includinglipogenesis andcholesterogenesis.It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Flow Cytometry: 1-3ug/1x10^6 cells, Immunofluorescence: 2-4 µg/mL

UniProt

P53396

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant protein (amino acids M1-I180) was used as the immunogen for the ATP Citrate Lyase antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

5I2

Applications

WB, IHC-P, FACS, IF

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the ATP Citrate Lyase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ATP Citrate Lyase antibody is available for research use only.

Storage Conditions

After reconstitution, the ATP Citrate Lyase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ATP Citrate Lyase antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human MCF7 cells with ATP Citrate Lyase antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.