APOBEC3G Antibody

APOBEC3G (apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G) is a human enzyme encoded by the APOBEC3G gene. This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Flow Cytometry: 1-3ug/1x10^6 cells, Immunofluorescence: 2-4 µg/mL

UniProt

Q9HC16

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human protein (amino acids E191-N384) was used as the immunogen for the APOBEC3G antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

6C2

Applications

WB, IHC-P, FACS, IF

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the APOBEC3G antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This APOBEC3G antibody is available for research use only.

Storage Conditions

After reconstitution, the APOBEC3G antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the APOBEC3G antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human MCF7 cells with APOBEC3G antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.