SMARCAD1 Antibody

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 is a protein that in humans is encoded by the SMARCAD1 gene. It is mapped to 4q22.3. This gene encodes a member of the SNF subfamily of helicase proteins. The encoded protein plays a critical role in the restoration of heterochromatin organization and propagation of epigenetic patterns following DNA replication by mediating histone H3/H4 deacetylation. Mutations in this gene are associated with adermatoglyphia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3 µg/mL, Immunoprecipitation: 2ug antibody/500ug lysate, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9H4L7

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids D827-T1002 from the human protein were used as the immunogen for the SMARCAD1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF/ICC, FACS, IP, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

Store the SMARCAD1 antibody at -20oC.

Limitations

This SMARCAD1 antibody is available for research use only.

Storage Conditions

Store the SMARCAD1 antibody at -20°C.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the SMARCAD1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of human 1) K562, 2) HL-60, 3) U-2 OS, 4) U-87 MG, 5) HepG2, 6) ThP-1 and 7) A549 lysate with SMARCAD1 antibody. Predicted molecular weight ~117 kDa but can be observed at up to ~150 kDa.