EIF4E Antibody
EIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1:100-1:5000
UniProt
P06730
Host
Rabbit
Reactivity
Human
Immunogen
A synthetic peptide specific to human EIF4E was used as the immunogen for the EIF4E antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
AFHI-5
Type
Recombinant
Applications
WB
Purity
Affinity purified
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the EIF4E antibody at -20oC.
Limitations
This EIF4E antibody is available for research use only.
Storage Conditions
Store the EIF4E antibody at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the EIF4E antibody should be determined by the researcher.
Image Legend
Western blot testing of human HEK293 cell lysate with EIF4E antibody. Predicted molecular weight ~27 kDa.
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