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CK18 Antibody / Cytokeratin 18

KRT18 encodes the type I intermediate filament chain keratin 18. Cytokeratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:100-1:500, Immunofluorescence/Immunocytochemistry: 1:50-1:200

UniProt

P05784

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide specific to human Cytokeratin 18 / KRT18 was used as the immunogen for the CK18 antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

GBF-11

Type

Recombinant

Applications

IHC-P, IF/ICC, WB

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the CK18 antibody at -20oC.

Limitations

This CK18 antibody is available for research use only.

Storage Conditions

Store the CK18 antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the CK18 antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE mouse liver tissue with CK18 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

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