Phospho-EIF2S1 Antibody (pS51)

EIF2S1 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:200

UniProt

P05198

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide specific to human EIF2S1 (surrounding phosphorylated S51) was used as the immunogen for the phospho-EIF2S1 antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

IO-5

Type

Recombinant

Applications

IHC-P, WB

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the phospho-EIF2S1 antibody (pS51) at -20oC.

Limitations

This phospho-EIF2S1 antibody (pS51) is available for research use only.

Storage Conditions

Store the phospho-EIF2S1 antibody (pS51) at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the phospho-EIF2S1 antibody (pS51) should be determined by the researcher.

Image Legend

Western blot testing of lysate from human HeLa cells 1) treated with Calyculin A and 2) untreated, with phospho-EIF2S1 antibody (pS51) . Predicted molecular weight ~36 kDa.