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MPO Antibody / Myeloperoxidase

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:2000

UniProt

P05164

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide specific to human Myeloperoxidase was used as the immunogen for the MPO antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

AOCF-13

Type

Recombinant

Applications

WB

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the MPO antibody at -20oC.

Limitations

This MPO antibody is available for research use only.

Storage Conditions

Store the MPO antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the MPO antibody should be determined by the researcher.

Image Legend

Western blot testing of human HL60 cell lysate with MPO antibody. Expected molecular weight: 59-64 kDa (alpha chain, may be observed at higher molecular weights due to glycosylation), 150+ kDa (glycosylated mature form) .

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