Acetyl-Histone H2A Antibody (Lys9)

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:500-1:1000

UniProt

P04908

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A synthetic peptide specific to human Histone H2A (surrounding acetylated lysine 9) was used as the immunogen for the Histone H2A antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

DEB-8

Type

Recombinant

Applications

IHC-P, WB

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the acetyl-Histone H2A Antibody (Lys9) at -20oC.

Limitations

This acetyl-Histone H2A Antibody (Lys9) is available for research use only.

Storage Conditions

Store the acetyl-Histone H2A Antibody (Lys9) at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the acetyl-Histone H2A Antibody (Lys9) should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of lysate from human HeLa cells treated with Trichostatin A, with acetyl-Histone H2A Antibody (Lys9) . Predicted molecular weight ~14 kDa.