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Apolipoprotein B Antibody / APOB

The APOB gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 (~240 kDa) and apoB-100 (~516 kDa) : the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:2000

UniProt

P04114

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide specific to human Apolipoprotein B / APOB was used as the immunogen for the Apolipoprotein B antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

AOIH-1

Type

Recombinant

Applications

WB

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the Apolipoprotein B antibody at -20oC.

Limitations

This Apolipoprotein B antibody is available for research use only.

Storage Conditions

Store the Apolipoprotein B antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Apolipoprotein B antibody should be determined by the researcher.

Image Legend

Western blot testing of human serum lysate with Apolipoprotein B antibody. Predicted molecular weight ~516 kDa.

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