Phospho-c-Raf Antibody (pS621)
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2) . [UniProt]
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1:500-1:2000
UniProt
P04049
Host
Rabbit
Reactivity
Human
Immunogen
A synthetic peptide specific to human Raf1 (surrounding pS621) was used as the immunogen for the phospho-c-Raf antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
IOF-18
Type
Recombinant
Applications
WB
Purity
Affinity purified
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the phospho-c-Raf antibody at -20oC.
Limitations
This phospho-c-Raf antibody is available for research use only.
Storage Conditions
Store the phospho-c-Raf antibody at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the phospho-c-Raf antibody should be determined by the researcher.
Image Legend
Western blot testing of human HeLa cells 1) untreated and 2) treated with LP, with phospho-c-Raf antibody. Predicted molecular weight ~73 kDa.
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