Phospho-ER alpha Antibody (pS118)

Estrogen receptor alpha is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:200, Immunofluorescence: 1:50-1:200

UniProt

P03372

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide specific to human ER alpha / ESR1 (surrounding pS118) was used as the immunogen for the phospho-ER alpha antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

EIG-5

Type

Recombinant

Applications

WB, IF, IHC-P

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the phospho-ER alpha antibody at -20oC.

Limitations

This phospho-ER alpha antibody is available for research use only.

Storage Conditions

Store the phospho-ER alpha antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the phospho-ER alpha antibody should be determined by the researcher.

Image Legend

Western blot testing of human MCF7 cells 1) untreated and 2) treated with b-Estradiol and EGF, with phospho-ER alpha antibody. Predicted molecular weight ~66 kDa.