Phospho-Histone H2B Antibody

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:1000

UniProt

P02293

Host

Rabbit

Reactivity

Yeast

Immunogen

A synthetic peptide specific to yeast Histone H2B (surrounding pT129) was used as the immunogen for the phospho-Histone H2B antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

DEI-8

Type

Recombinant

Applications

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the phospho-Histone H2B antibody at -20oC.

Limitations

This phospho-Histone H2B antibody is available for research use only.

Storage Conditions

Store the phospho-Histone H2B antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the phospho-Histone H2B antibody should be determined by the researcher.

Image Legend

Western blot testing of lysate from Methyl methanesulfonate-treated Saccharomyces cerevisiae with phospho-Histone H2B antibody. Predicted molecular weight ~14 kDa.