TIMM17A Antibody

Mitochondrial import inner membrane translocase subunit Tim17-A is an enzyme that in humans is encoded by the TIMM17A gene. It is mapped to 1q32.1. TIM17 is a conserved suppressor of mtDNA instability, and overexpression of TIMM17A can prevent mtDNA loss in a human cellular model of mitochondrial disease.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Immunofluorescence/Immunocytochemistry: 2-4 µg/mL

UniProt

Q99595

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids LFSMIDCSMVQVRGKED from the human protein were used as the immunogen for the TIMM17A antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF/ICC

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the TIMM17A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TIMM17A antibody is available for research use only.

Storage Conditions

After reconstitution, the TIMM17A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TIMM17A antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of human 1) placenta, 2) K562, 3) T-47D, 4) Caco-2, 5) HeLa, 6) ThP-1, 7) A431 and 8) A549 lysate with TIMM17A antibody at 0.5ug/ml. Predicted molecular weight ~18 kDa.