TMEM199 Antibody

TMEM199 encodes a protein homologous to the yeast V-ATPase assembly factor Vma12 and appears to be involved in Golgi homeostasis. The protein encoded by this gene has been observed to localize to the endoplasmic reticulum (ER) -Golgi intermediate compartment (ERGIC) and coat protein complex I (COPI) in some human cells. Defects in this gene are a cause of congenital disorder of glycosylation, type IIp. By genomic sequence analysis, the TMEM199 gene is mapped to chromosome 17q11.1.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q8N511

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids E20-H129 from the human protein were used as the immunogen for the TMEM199 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the TMEM199 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TMEM199 antibody is available for research use only.

Storage Conditions

After reconstitution, the TMEM199 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TMEM199 antibody should be determined by the researcher.

Image Legend

Western blot testing of human 1) HeLa, 2) placenta, 3) Caco-2, 4) T-47D, 5) U-87 MG, 6) K562, 7) U-2 OS and 8) PC-3 lysate with TMEM199 antibody at 0.5ug/ml. Predicted molecular weight ~23 kDa.