KBTBD2 Antibody

Kelch repeat and BTB domain containing 2 (KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85 alpha-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85 alpha protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling. However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization. In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity and insulin sensitivity.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunocytochemistry/Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q8IY47

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids QYDLELDRWSLRQHISERVLWDLGRDFR from the human protein were used as the immunogen for the KBTBD2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF/ICC, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the KBTBD2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This KBTBD2 antibody is available for research use only.

Storage Conditions

After reconstitution, the KBTBD2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the KBTBD2 antibody should be determined by the researcher.

Image Legend

Western blot testing of human 1) HeLa, 2) placenta, 3) K562, 4) U-2 OS, 5) ThP1 and 6) A549 lysate with KBTBD2 antibody at 0.5ug/ml. Predicted molecular weight ~71 kDa.