PPA1 Antibody / Inorganic pyrophosphatase

PPA1 is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. By radiation hybrid analysis, the PPA1 gene is mapped to chromosome 10q21.3.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q15181

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids E7-N236 from the human protein were used as the immunogen for the PPA1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the PPA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PPA1 antibody is available for research use only.

Storage Conditions

After reconstitution, the PPA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PPA1 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of human 1) PC-3, 2) Caco-2, 3) HEK293, 4) K562 and 5) U-2 OS lysate with PPA1 antibody at 0.5ug/ml. Predicted molecular weight: ~33 kDa.