NFIA Antibody
Nuclear factor 1 A-type is a protein that in humans is encoded by the NFIA gene. Nuclear factor I (NFI) proteins constitute a family of dimeric DNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiplegenes, differential splicing, and heterodimerization.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow Cytometry: 1-3ug/10^6 cells, Immunofluorescence: 2-4 µg/mL
UniProt
Q12857
Host
Mouse
Reactivity
Human
Immunogen
Amino acids AYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSGVFS from the human protein were used as the immunogen for the NFIA antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
16H11
Applications
WB, IHC-P, FACS, IF
Purity
Purified
Format
Purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
Limitations
This NFIA antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the NFIA antibody should be determined by the researcher.
Image Legend
IHC staining of FFPE human tonsil with NFIA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Frequently Asked Questions
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