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NFIA Antibody

Nuclear factor 1 A-type is a protein that in humans is encoded by the NFIA gene. Nuclear factor I (NFI) proteins constitute a family of dimeric DNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiplegenes, differential splicing, and heterodimerization.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow Cytometry: 1-3ug/10^6 cells, Immunofluorescence: 2-4 µg/mL

UniProt

Q12857

Host

Mouse

Reactivity

Human

Immunogen

Amino acids AYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSGVFS from the human protein were used as the immunogen for the NFIA antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

16H11

Applications

WB, IHC-P, FACS, IF

Purity

Purified

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the NFIA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NFIA antibody is available for research use only.

Storage Conditions

After reconstitution, the NFIA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NFIA antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human tonsil with NFIA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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