CD47 Antibody / Integrin Associated Protein / IAP

CD47, also known as IAP or MER6, is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 gene belongs to the immunoglobulin superfamily. This gene is mapped to 3q13.12. CD47 gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow Cytometry: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q08722

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids Q19-E323 from the human protein were used as the immunogen for the CD47 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the CD47 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CD47 antibody is available for research use only.

Storage Conditions

After reconstitution, the CD47 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CD47 antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

Flow cytometry testing of human PBM cells with CD47 antibody at 1ug/10^6 cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= CD47 antibody.