Caveolin-3 Antibody / CAV3

Caveolin-3 is a protein that in humans is encoded by the CAV3 gene. This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD) . Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P56539

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids M1-D55 from the human protein were used as the immunogen for the Caveolin-3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Caveolin-3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Caveolin-3 antibody is available for research use only.

Storage Conditions

After reconstitution, the Caveolin-3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Caveolin-3 antibody should be determined by the researcher.

Location

Cytoplasmic, plasma membrane

Image Legend

Western blot testing of 1) human placenta, 2) U-87 MG, 3) human HeLa, 4) rat stomach, 5) rat testis, 6) mouse stomach and 7) mouse testis lysate with Caveolin-3 antibody at 0.5ug/ml. Predicted molecular weight: ~17 kDa but routinely observed at 20~25 kDa.