BAG6 Antibody / BAT3

Large proline-rich protein BAG6 is a protein that in humans is encoded by the BAG6 gene. It is mapped to 6p21.33. This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P46379

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids D15-A88 from the human protein were used as the immunogen for the BAG6 Antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the BAG6 Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This BAG6 Antibody is available for research use only.

Storage Conditions

After reconstitution, the BAG6 Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the BAG6 Antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of 1) human HeLa, 2) human ThP-1, 3) human U-87 MG, 4) rat brain, 5) rat heart, 6) rat lung, 7) rat liver, 8) mouse brain, 9) mouse heart and 10) mouse lung lysate with BAG6 antibody at 0.5ug/ml. Predicted molecular weight ~119 kDa but observed at 150-170 kDa.