Wee1 Antibody
Wee1 is a nuclear kinase belonging to the Ser/Thr family of protein kinases in the fission yeast Schizosaccharomyces pombe (S. pombe) . I t is mapped to 11p15.4. This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P30291
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids ELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDR from the human protein were used as the immunogen for the Wee1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS, IHC-P
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
Limitations
This Wee1 antibody is available for research use only.
Storage Conditions
Product Datasheet
https://www.nsjbio.com/tds-pdf/wee1-antibody-rq4901
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Wee1 antibody should be determined by the researcher.
Image Legend
IHC staining of FFPE human breast carcinoma with Wee1 antibody. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.
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