Phospho-c-Myc Antibody (pT58)

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:2000

UniProt

P01106

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide specific to human c-Myc (surrounding pT58) was used as the immunogen for the phospho-c-Myc antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

FOC-13

Type

Recombinant

Applications

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the phospho-c-Myc antibody at -20oC.

Limitations

This phospho-c-Myc antibody is available for research use only.

Storage Conditions

Store the phospho-c-Myc antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the phospho-c-Myc antibody should be determined by the researcher.

Image Legend

Western blot testing of lysate from 1) untreated and 2) Calyculin A and Okadaic Acid-treated human HeLa cells with phospho-c-Myc antibody. Theoretical molecular weight: ~50 kDa but routinely observed at 50~70 kDa.