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EGFR Antibody

EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:5000-1:10000, Immunohistochemistry (FFPE) : 1:50-1:200, Immunofluorescence: 1:50-1:200

UniProt

P00533

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide specific to human EGFR was used as the immunogen for the EGFR antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

AEF-5

Type

Recombinant

Applications

IHC-P, WB, IF

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the EGFR antibody at -20oC.

Limitations

This EGFR antibody is available for research use only.

Storage Conditions

Store the EGFR antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the EGFR antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of human HeLa cell lysate with EGFR antibody. Expected molecular weight: 134-170 kDa depending on glycosylation level.

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