ATG7 Antibody
ATG7 is an E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. [UniProt]
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1:10000-1:50000
UniProt
O95352
Host
Rabbit
Reactivity
Human
Immunogen
A synthetic peptide specific to human ATG7 was used as the immunogen for the ATG7 antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
FCA-1
Type
Recombinant
Applications
WB
Purity
Affinity purified
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the ATG7 antibody at -20oC.
Limitations
This ATG7 antibody is available for research use only.
Storage Conditions
Store the ATG7 antibody at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the ATG7 antibody should be determined by the researcher.
Image Legend
Western blot testing of human HepG2 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.
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