ATG12 Antibody
Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. [UniProt]
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1:500-1:2000
UniProt
O94817
Host
Rabbit
Reactivity
Human
Immunogen
A synthetic peptide specific to human ATG12 was used as the immunogen for the ATG12 antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
ABID-1
Type
Recombinant
Applications
WB
Purity
Affinity purified
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the ATG12 antibody at -20oC.
Limitations
This ATG12 antibody is available for research use only.
Storage Conditions
Store the ATG12 antibody at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the ATG12 antibody should be determined by the researcher.
Image Legend
Western blot testing of human HepG2 lysate with ATG12 antibody. Expected molecular weight: ~15 kDa (ATG12) and 50-60 kDa (ATG12-ATG5 complex) .
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