Brd4 Antibody

Bromodomain-containing protein 4 is a chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:200, Immunofluorescence/Immunocytochemistry: 1:50-1:200

UniProt

O60885

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide specific to human Brd4 was used as the immunogen for the Brd4 antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

DBH-2

Type

Recombinant

Applications

IF/ICC, IHC-P, WB

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the Brd4 antibody at -20oC.

Limitations

This Brd4 antibody is available for research use only.

Storage Conditions

Store the Brd4 antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Brd4 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of human HeLa cell lysate with Brd4 antibody. Predicted molecular weight ~156 kDa (long form, can be observed at 200+ kDa) and ~81 kDa (short form) .