Phospho-Chk1 Antibody (pS296)
The protein encoded by CHEK1 belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. [RefSeq]
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1:500-1:2000
UniProt
O14757
Host
Rabbit
Reactivity
Human
Immunogen
A synthetic peptide human Chk1 / CHEK1 (surrounding pS296) was used as the immunogen for the phospho-Chk1 antibody (pS296) .
Clonality
Monoclonal
Isotype
IgG
Clone
FEO-3
Type
Recombinant
Applications
WB
Purity
Affinity purified
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the phospho-Chk1 antibody (pS296) at -20oC.
Limitations
This phospho-Chk1 antibody (pS296) is available for research use only.
Storage Conditions
Store the phospho-Chk1 antibody (pS296) at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the phospho-Chk1 antibody (pS296) should be determined by the researcher.
Image Legend
Western blot testing of lysate from human HEK293 cells treated with Calyculin with phospho-Chk1 antibody (pS296) . Predicted molecular weight ~54 kDa.
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
Protein G Coated - 96 well plates - 8 Well Breakable Strips on single well holding frame - Clear PS
CD15 / FUT4 (Reed-Sternberg Cell Marker) (FUT4/1478R), CF488A conjugate, 0.1mg/mL
