Phospho-Chk1 Antibody (pS296)

The protein encoded by CHEK1 belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:2000

UniProt

O14757

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide human Chk1 / CHEK1 (surrounding pS296) was used as the immunogen for the phospho-Chk1 antibody (pS296) .

Clonality

Monoclonal

Isotype

IgG

Clone

FEO-3

Type

Recombinant

Applications

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the phospho-Chk1 antibody (pS296) at -20oC.

Limitations

This phospho-Chk1 antibody (pS296) is available for research use only.

Storage Conditions

Store the phospho-Chk1 antibody (pS296) at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the phospho-Chk1 antibody (pS296) should be determined by the researcher.

Image Legend

Western blot testing of lysate from human HEK293 cells treated with Calyculin with phospho-Chk1 antibody (pS296) . Predicted molecular weight ~54 kDa.