CHK1 Antibody / CHEK1
The protein encoded by CHEK1 belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. [RefSeq]
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1:500-1:2000
UniProt
O14757
Host
Rabbit
Reactivity
Human, Rat
Immunogen
A synthetic peptide specific to human CHK1 / CHEK1 was used as the immunogen for the CHK1 antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
AAF-3
Type
Recombinant
Applications
WB
Purity
Affinity purified
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the CHK1 antibody at -20oC.
Limitations
This CHK1 antibody is available for research use only.
Storage Conditions
Store the CHK1 antibody at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the CHK1 antibody should be determined by the researcher.
Image Legend
Western blot testing of 1) human K562 and 2) rat PC-12 cell lysate with CHK1 antibody. Predicted molecular weight ~54 kDa.
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