P62 Antibody / SQSTM1

SQSTM1 (Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2 (SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The International Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al. (1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al. (1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE and Frozen) : 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Immunocytochemistry: 1-2 µg/mL, Western blot: 0.5-1 µg/mL

UniProt

Q13501

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids DEDGDLVAFSSDEELTMAMSYVKDDIFR of human p62 were used as the immunogen for the p62 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, IHC-F, IF, ICC, WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the p62 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This p62 antibody is available for research use only.

Storage Conditions

After reconstitution, the p62 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the p62 antibody should be determined by the researcher.

Location

Cytoplasmic, membranous, nuclear

Image Legend

Immunofluorescent staining of human lung cancer tissue with p62 antibody (red) at 1ug/ml and DAPI counterstain. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.