SRI Antibody / Sorcin

Sorcin is a protein that in humans is encoded by the SRI gene. It is mapped to 7q21.1. This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL

UniProt

P30626

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids TVDPQELQKALTTMGFRLSPQAVNSIAKRY were used as the immunogen for the SRI antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the SRI antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This SRI antibody is available for research use only.

Storage Conditions

After reconstitution, the SRI antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the SRI antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of human 1) placenta, 2) U-2 OS, 3) A431, 4) PC-3, 5) HL-60, 6) K562, 7) Caco-2, 8) rat lung and 9) mouse lung lysate with SRI antibody at 0.5ug/ml. Predicted molecular weight ~22 kDa, routinely observed at 22-29 kDa.